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ND-One-Spectrophotometer

NanoDrop™ One spectrometer

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The NanoDrop One Spectrophotometer is an advanced microvolume UV-Vis spectrophotometer specifically designed for rapid and precise analysis of DNA, RNA, and protein samples. Manufactured by Thermo Scientific, this state-of-the-art instrument combines innovative technology with user-friendly design, making it indispensable for laboratories requiring high accuracy and efficiency.

Whether you need a UV-Vis spectrophotometer for DNA and protein quantification or a device for assessing nucleic acid purity, the NanoDrop One offers unparalleled performance and versatility.

Key Features of ND One Spectrophotometer

1. Acclaro Sample Intelligence Technology

  1. Identify contaminants such as RNA in dsDNA or phenol in RNA samples within seconds.
  2. Receive instant feedback on sample quality via alerts, ensuring robust downstream applications.
  3. Rely on accurate concentration measurements through embedded sensor and digital image analysis.

2. Standalone Design for Convenience

  1. The ergonomic, integrated Android™ tablet interface eliminates the need for an external computer.
  2. High-resolution touchscreen enhances usability and provides a smooth, intuitive experience.
  3. Auto-range pathlength technology facilitates accurate measurements of highly concentrated samples (up to 27,500 ng/µL dsDNA) without dilution.

3. Advanced Data Management and Connectivity

  1. Multiple options for data transfer: Wi-Fi, Ethernet, or USB connectivity to PC, or save directly to the Thermo Fisher Cloud for 24/7 data access.
  2. Export data in detailed formats, including spectral graphs, and organize experiments efficiently with NanoDrop One PC software.
  3. Collaborate easily by sharing data with colleagues through Thermo Fisher Cloud.

4. Wide Spectral Range for Versatile Applications

With a wavelength range of 190 to 850 nm, the NanoDrop One supports various applications, including:

  1. DNA and RNA quantification (260 nm)
  2. Protein analysis (280 nm)
  3. Peptides (205 nm)
  4. Colorimetric protein assays (e.g., BCA at 562 nm, Bradford at 595 nm, Pierce 660 at 660 nm)
  5. Gold nanoparticle studies (520 nm)
  6. Optical density measurements (600 nm)

5. Enhanced Measurement Capabilities

  1. Patented** sample-retention system ensures accurate measurements for low-volume samples.
  2. Analyze concentration ranges from 2.0–27,500 ng/µL dsDNA and 0.06–820 mg/mL BSA without sample dilution.
  3. Auto-Blank and Auto-Measure functions improve workflow efficiency, reducing user intervention.

6. Optional 21 CFR Part 11 Compliance

For labs requiring regulatory compliance, the NanoDrop One can be equipped with an optional Thermo Scientific Security Suite, which provides:

  1. User account access with permissions.
  2. Comprehensive audit trails and electronic signatures.
  3. Automated data backup for secure documentation.

7. NanoDrop OneC: Added Versatility

  1. Includes both pedestal and cuvette positions to expand experimental flexibility.
  2. Perform kinetics experiments, measure dilute samples, or analyze bacterial culture optical density.
  3. Cuvette mode features temperature control and stirring capabilities.

 

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Applications of NanoDrop One Spectrophotometer

  1. Nucleic Acid Quantification

    1. Accurately measure DNA, RNA, and oligonucleotide concentrations.
    2. Evaluate sample purity with A260/A280 and A260/A230 ratios.
  2. Protein Analysis

    1. Quantify protein concentrations using methods like Bradford, BCA, and Lowry assays.
    2. Detect and analyze interfering substances to ensure sample integrity.
  3. Research and Diagnostics

    1. Ideal for biopharma quality control, academic research, and clinical diagnostics.
    2. Supports workflows in genomics, proteomics, and molecular biology.
  4. Rapid Contaminant Identification

    1. Identify and quantify contaminants such as phenol, proteins, and salts for improved data quality.

Benefits of the NanoDrop One Spectrophotometer

  1. Precision and Reliability: High accuracy across a broad concentration range (2.0 to 27,500 ng/µL for dsDNA and up to 820 mg/mL for proteins).
  2. Compact and Portable: Designed for easy integration into any workspace.
  3. Comprehensive Wavelength Range: Full-spectrum analysis from 190 to 850 nm for a wide variety of applications.
  4. Efficiency: Results in just 8 seconds streamline laboratory processes.
  5. Durability: Advanced components like the xenon flash lamp ensure long-lasting performance.

Technical Specifications of NanoDrop One Spectrophotometer

Feature Details
Voltage Options 230VAC, 60Hz; 460VAC, 60Hz; 220VAC, 50Hz; 380VAC, 50Hz (3 Phase)
Power Consumption 4.0kW
Motor Power 5Hp [3.7kW]
Sound Rating 78dBA (no load)
Recirculation Tank Capacity 22 gallons [83 liters]
Lighting Two LED Goosenecks
Abrasive Wheel Diameter Maximum 12 inches [305mm]; Arbor size 1.25 inches [32mm]
Cutting Chamber Dimensions 36 W x 22 D x 8 H inches [914 x 558 x 203mm]
Auto Y-Axis Feed Rate 0.7-5 in/min [0.3-2mm/sec]
Maximum Cut Diameter 4.375 inches [110mm]
Wheel Speed 2600rpm @ 50/60Hz; 8200 surface ft/min [2700 surface m/min]
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